RE: [Histonet] H&E Staining

From:Shelly Coker

If you are using an alcohol soluble eosin, I recommend removing the 70% alcohol from your procedure.  Go straight to 95% after the eosin, then a couple changes of 100%.  You can adjust the timing in the 95% alcohol to get it just right b/c the 95% alcohol does the differentiating of the eosin staining.  The longer in the 95%, the less pink staining there will be.  Alot of the timing depends on the type of eosin you are using.  
  Happy staining!

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