Re: [Histonet] RE: Using the best Retic Silver Stain for Bone marrow biopsies

From:Rene J Buesa

Medea J.:
  In my experience, after trying many different procedures, I found that the best and more consistent is Gomori's ammoniacal silver method.
  René J.

"McGraw, Medea J"  wrote:
I am searching for an optimal method for providing the best Retic silver stain, aside from using automation and kits, on bone marrow biopsies. Currently, we have been using the Wilder's technique and ran into inconsistencies in the past 5 months or so. Is there a better method than your lab is currently using that you would care to share with me to try? I have also tried the Gridley's and the results are good fibers but very muddy. We are currently using a Nexus to help us "get by" but the kits are $$$ and it's just a temporary fix. I am interested in all methods (microwave or not). I would appreciate any suggestions. 

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