Hello, histonetters:Has anyone done cytospin slide staining before? What antigen retrieval method you used?I am staining bronchoalveolar lavage fluid slides with anti-active caspase3 and none of my experimental and control slides showed positive.I am wondering if that is real or I did it wrong since I don't have any positive control slides for this antibody and I never did this kind of staining before.Any suggestion will be greatly appreciated!Have a nice day.Dahui YouLSU
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