RE: [Histonet] Ventana Benchmark Problems

From:"Tarango, Mark"

I'm with you on this one!  The kits and reagents are very expensive and
the hit and miss staining drives me nuts.  I have to re-stain the same
slide over and over again because the control stained and the patient
tissue didn't have any reactive elements or there was no staining at

I also can't stand that the instrument won't stain slides because I run
out of something like EZprep, which just dewaxes the slide.  If I run a
slide down to water and put it on the instrument why should it need
EZrep? I mean, there is no paraffin on the slide and the protocol has
been modified accordingly, so I think the stupid thing should run.  

Another thing, what is the deal with the expiration dates?  Their kits
are the most expensive and expire the soonest.  After they expire,
they're dead.  Not even for research purposes or if there were an
emergency could you get the machine to run with an expired kit. 

Mark Adam Tarango HT(ASCP)
Histology/Immunohistochemistry Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV  89135
Direct Line (702) 822-5112
Fax (702) 939-7663


-----Original Message-----
[] On Behalf Of Joe
Sent: Wednesday, October 18, 2006 4:48 PM
To: Bates Robert R Maj MIL USAF 96 MDSS/SGSAH;
Subject: Re: [Histonet] Ventana Benchmark Problems

yes and it's not even Friday yet.
Hey Doc,
we have 3 of these "great" machines and it drives us nuts also. Make it
point, in fact, demand that an engineering rep come out. We have had
replaced such as the vortex mixer, heating pads, the entire carousel
times, yet for some strange reason, we keep getting more and more.
    The big thing now is we switched to their polymer detection kit and
talk about fits. All our slides have a hit and miss on staining
peroxidase. Our rabbit and mouse negative controls stain as brightly as
primaries. Yes, we had two "wiz" kids out and I was not impressed. A
specialist is coming to my lab to figure out the problem. You see me
my breath.
    I told the group not to go ahead with the Ventana machines, but they

blew me off. Said I had an attitude problem because I was screwed over
I was at Wilford Hall. Gee, maybe I was on to something. Now, we are in
deep we'll never get out.
    If it was my lab, I would have went with Biocare's Nemesis, but like
said, it's not my lab.
    If you can change machines, I would. Save yourself a lot of headache
    Now, I expect all the Ventana reps and their lawyers to contact my
tomorrow. Go ahead, make my day. Save yourself the phone call. Whatever
said here, I will say in court too.
    Of course, the opinion expressed by me is not the opinion of my 
pathologists, CEO, their lawyers, their wives, off spring, their off
and their dogs and cats.

Histology Manager
Pathology Reference Lab
San Antonio, TX
----- Original Message ----- 
From: "Bates Robert R Maj MIL USAF 96 MDSS/SGSAH" 

Sent: Wednesday, October 18, 2006 2:23 PM
Subject: [Histonet] Ventana Benchmark Problems

> Hello,
> We have a Ventana Benchmark that has been giving my histotechs fits. 
> There
> are times when the staining is hit or miss.  Other times the controls
> not stain properly but the specimen tissue does or visa versa.  We
> troubleshot the machine numerous times without success.  We call the
> line and do the things they recommend and it 'may' work for a while
> then
> it reverts back to the hit or miss schedule.  We have tried random
> sections that have know positive properties (such as smooth muscle
> stained with smooth muscle actin) and even observed internal tissue 
> controls
> to see if they react properly.  Has anyone else had this type of
> with this machine?  Any ideas or suggestions would be most welcome.
> Dr. Robert R. Bates Jr
> Florida
> _______________________________________________
> Histonet mailing list

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