[Histonet] Antibody dilutions and temperature
Hello all,
I have noticed there are different diluents used for various antibodies; I
have seen PBS, PBS plus albumin, and PBS/albumin/Tween.
What is the difference between using one or the other of these? Also, how
does incubation temperature play a role in the choice? Thanks for your
answer, Tim :). Actually I would like to hear anyone's opinion since just
about everyone here is an IHC or IF expert. Enjoy the weekend!
Mel
Melville B. Vaughan, Ph. D.
Assistant Professor
Department of Biology
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm
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