[Histonet] Antibody dilutions and temperature


Hello all,
I have noticed there are different diluents used for various antibodies; I 
have seen PBS, PBS plus albumin, and PBS/albumin/Tween. 
What is the difference between using one or the other of these? Also, how 
does incubation temperature play a role in the choice? Thanks for your 
answer, Tim :). Actually I would like to hear anyone's opinion since just 
about everyone here is an IHC or IF expert. Enjoy the weekend!

Melville B. Vaughan, Ph. D.
Assistant Professor
Department of Biology
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
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