I don't recall this question, but I would be interested in how you get 
around the problem of epidermal pigmentation if you are looking for 
protein expression in the epidermis; do you bleach the tissue (and if so, 
does that affect antigenicity of the proteins), or can you choose a 
detection chromogen that has a wild color, like a Barney purple color? 
Thanks for your help.
Mel
Melville B. Vaughan, Ph. D.
Assistant Professor
Department of Biology
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm
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