Re: [Histonet] Bone Marrow Biopsies

From:Rene J Buesa (by way of histonet)

What you do at the sample site depends on what the research is all about.
We used to do the following:
1- at the site  we took 4 to 5 smears and fixed the core in neutral (but
really neutral) buffered formalin, inside a glass container (2-3 oz.).
2- after 4-6 hours the biosy was places in EDTA and was "decalcified" for
4-6 hours and latter processed, cut, stained, etc. as usual.
3- the liquid and remanent blood contained in the vial was filtered through
paper filter, placed in cassette and processed in the same way as the
4- both decalcified biopsy and filtrate of aspirate were cut as thin as
possible, except for the slides used for iron or reticulin stain, that were
cut at 7 um
Hope this will help!
Rene J.

Cheryl Crowder  wrote:
Hi all - I have a residen whose research will include doing bone marrow
biopsies. Could someone help me with protocols for bone marrows. Vet Med
here does not routinely do bone marrows and the results are spotty. What I
am looking for is what you do at the point of biopsy, as make smears, fix
some, etc., what fixative do you use, do you decal the BM and in what? All
that "stuff". If anyone can help me, it would probably be better to e-mail
me directly. Thank you,

Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Anatomic Pathology
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Skip Bertman Drive
Baton Rouge, LA 70803

FAX: 225-578-9720

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