Re: [Histonet] IHC withquantum dots

From:"LaCinda Burchell"

Dear Baowei Peng,
  Can you tell us why we can't co-localize?  Cindy B

LaCinda Burchell, BA, AS, HT(ASCP)
University of Wisconsin-Madison, Medical School
Asthma and Allergy Research IHC Lab
600 Highland Ave.  CSC  K4/913
Madison, Wisconsin  53792

Phone: 608-262-3518
FAX:     608-263-3746

>>> Baowei Peng  10/14/2004 2:20:32 AM >>>

We have a fellow in our lab who worked on QDs (quantum dots).
So I have learned that most QDs can be excitated under wave length
about between 400-600nm.
It means that you can use one certain wave length to excitate several
different QDs which are distinctive in emission wave length (thus
different in color). But the drawback is that you can not use two or
more QDs in order to see colocalization such as nucleus and membrane
molecules of the same cell. 


Baowei Peng
1954 Huashan Road
Pharmacy School
Shanghai Jiaotong University
Shanghai, 200030

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