Re: [Histonet] IHC withquantum dots
Dear Baowei Peng,
Can you tell us why we can't co-localize? Cindy B
LaCinda Burchell, BA, AS, HT(ASCP)
University of Wisconsin-Madison, Medical School
Asthma and Allergy Research IHC Lab
600 Highland Ave. CSC K4/913
Madison, Wisconsin 53792
>>> Baowei Peng 10/14/2004 2:20:32 AM >>>
We have a fellow in our lab who worked on QDs (quantum dots).
So I have learned that most QDs can be excitated under wave length
about between 400-600nm.
It means that you can use one certain wave length to excitate several
different QDs which are distinctive in emission wave length (thus
different in color). But the drawback is that you can not use two or
more QDs in order to see colocalization such as nucleus and membrane
molecules of the same cell.
1954 Huashan Road
Shanghai Jiaotong University
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