Hello Histonet,

I need some insight on GAG staining please. I've done different times, 
different pH's. I have explants grown in aragose at 0 weeks that are not 
suppose to stain but do, and real tissue that's suppose to stain red and 
turns out green.
I started with Lillie's (3rd ed.) procedure. Does anyone have a tried, 
true, and repeatable procedure? Is it my water, is it my staining dishes? 
Can dry stain go bad?
Wit's end and other things to do. Thank you very much for any help.  It's 
bovine tissue.
Mary Lou  


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