[Histonet] Bone marrow core biopsy handling

From:"Cazares, Ruth"

Hello again,


I am interested to know how bone marrow cores are handled out there in
histo-land.  In our lab we put the cores in a formic acid decalcifier
(which is supposed to fix the tissue as it decals according to the
company) as soon as we get it. It stays in for 2-3 hours depending on
the amount of bone it contains.  Then it is processed on the routine
overnight program for large tissues. We do not use a short program for
BM cores.  The H&E's are ok, I guess, but nothing compared to B-5
fixation.  We have gotten rid of all the mercury in our labs and I'm
wondering what are the alternatives that people are using?


Thanks again!


Ruth Cazares


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