clarification please

your routine immuno includes ?

looking for human cells in mouse?

your primary antibody is made against human tumor cells, what animal is the
antibody made in?

what do you use as a secondary antibody?

anita




                                                                           
             wen eng                                                       
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                                                                   Subject 
                                       [Histonet] human Ab on human tumor  
             10/26/2004 02:00                                              
             PM                                                            
                                                                           
                                                                           
                                                                           
                                                                           




Hi histonetters,

Currently I am doing IHC using human Ab on human tumor. I know it would
cause "messy" background. But this tumor was developed by injection human
tumor cell to nude mice. The results came out pretty good. I didn't use
conjugated primary Ab, but just traditional method. I think it's because
the tumor was developed in mice, the tissue would not contain endogenous
human serum that causes the "messy background", although the tumor is human
tumor. Am I right on this? Or my stain result just "fake"?
Hope I said it clearly. Could any expert give me help?

Thanks in advance!



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