[Histonet] (no subject)

From:Chris Jones

Dear all, I am hoping that someone can be of assistance.

I am trying to fix embryonic chick limb buds, to preserve as much as 
possible the spatial relations of the cells. A Karnovsky-like fixation 
(glutaraldehyde + paraformaldehyde, postfixed in osmium tetroxide) fixes 
really well, but apparently Karnovsky fixatives induce a fair bit of 
shrinkage. A 'better' fixative ie induces less shrinkage (osmium + 
glutaraldehyde as primary fixatives) has so far given lesser quality 
fixation. Does anybody know of a modification we can make (with confidence) 
to the osmium/glut fix that will improve things? Or does postfixation in 
osmium induce less shrinkage anyway?

I will be most appreciative of any nibbles. Many thanks, Chris.

Christopher Jones, PhD
Department of Anatomical Sciences
University of Adelaide
Ph. +61 8 8303 4526
Fax. +61 8 8303 4398
CRICOS Provider Number 00123M
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