[Histonet] (no subject)
Dear all, I am hoping that someone can be of assistance.
I am trying to fix embryonic chick limb buds, to preserve as much as
possible the spatial relations of the cells. A Karnovsky-like fixation
(glutaraldehyde + paraformaldehyde, postfixed in osmium tetroxide) fixes
really well, but apparently Karnovsky fixatives induce a fair bit of
shrinkage. A 'better' fixative ie induces less shrinkage (osmium +
glutaraldehyde as primary fixatives) has so far given lesser quality
fixation. Does anybody know of a modification we can make (with confidence)
to the osmium/glut fix that will improve things? Or does postfixation in
osmium induce less shrinkage anyway?
I will be most appreciative of any nibbles. Many thanks, Chris.
Christopher Jones, PhD
Department of Anatomical Sciences
University of Adelaide
ADELAIDE SOUTH AUSTRALIA 5005
Ph. +61 8 8303 4526
Fax. +61 8 8303 4398
CRICOS Provider Number 00123M
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