[Histonet] Getting stented sections to stay on slides.
I have posted this question before and now I am beside myself. I cut
5um sections of stented arteries and mount them on super frost ++
slides. I have tried other slides like Apes, polylysine etc but still
have no luck. I have floated out at 57*c and tried 70*c distilled
water baths, then floated onto acetone as well but still no luck. I
have incubated at 37*c overnight, 10 minutes at 60*c , 70*c for 2
hours but to no avail. I have had it as my sections are really good
and all I do now is lose them. Ever felt that way. When I first
started doing this work I got them to stay on this way ie pick up on
coated slides, incubate at 37*c overnight and deresin in 2x changes
of warm xylene at 37*C 15 minutes each. worked for a while. Is there
anyone out there experiencing the same kind of problems? Many thanks.
By the way I am using Mma Neil Hand`s method.
Cardiovascular Research Unit
University of Cape Town
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