[Histonet] Alcoholic eosin with floxien

From:"kevin williams"

Dear All:
I have a linistaner and want to change my eosin from floxinated, to alcoholic. The slides on my linistaner are only in the bath for 10 seconds can I expect to get the same degree of staining?
Why am I changing you ask? Because of water in my clearing agent.
I have licked the water problem for the moment, but I change my alcohols often and I have an evaporation plate to reduce atmospheric water and I have an adsorbent (I believe it is adsorbent) packet sitting in the end chamber to catch all the residual.
What I am looking for is to get some idea from people who have already got alcoholic eosin in there linistainer and give me some idea of what they use before it, 50% alcohol, 95% alcohol etc. I have 95% after my one bath of eosin and then 6 x 100% to my clearing agent.
All of this cost time and money. If I can get the alcoholic eosin to work I think this would save on time and money.
Thanks for your help
Yours faithfully:
A.Kevin Williams

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