Your water bath may be too high, neuro tissue tends to explode on a water 
bath that is higher than 38-40 degrees celcius.  Try cooling the bath down.
Joyce Edmonds
MUSC
Charleston, SC

--On Wednesday, October 08, 2003 12:26 PM -0400 LaTricia Faison 
 wrote:

> Does anyone know what may cause mouse brain to separate when put on the
> water bath?  The tissue was given to me after it had been in
> paraformaldehyde for about 7-8 months.  It was paraffin processed and
> shattered soon after touching the water bath.
>
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