[Histonet] Purple Haze.....
Kathleen:
Thanks for naming the amorphous blight that has been plaguing us
lately. This "Jimi Hendrix" artifact has been running 'round our brains
for the past few weeks. We have 1-started filtering the hematoxylin
each morning, 2-alternated between Sta-on, gelatin, and naked
waterbaths, 3-scrubbed the entire staining system, 4-decreased time in
microwave, ovens, for drying slides; and yet, we still find this on some
biopsies. Not always all the biopsies in a batch, this led us to ask GI
about collection procedures, and possible air-drying, they insist it's
not them. We've checked pH on water, accurately calibrated the bluing
and acid/water washes (we stain regressively), and just about run out of
ideas. I'm beginning to suspect the hematoxylin is leaving a
precipitate, or maybe the microtomy is inconsistent and ruining cell
integrity, or .... maybe it's terrorism! Any ideas out there?
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