[Histonet] Purple Haze.....

From:"Therersa Stegall"

   Thanks for naming the amorphous blight that has been plaguing us
lately.  This "Jimi Hendrix" artifact has been running 'round our brains
for the past few weeks.  We have 1-started filtering the hematoxylin
each morning, 2-alternated between Sta-on, gelatin, and naked
waterbaths, 3-scrubbed the entire staining system, 4-decreased time in
microwave, ovens, for drying slides; and yet, we still find this on some
biopsies.  Not always all the biopsies in a batch, this led us to ask GI
about collection procedures, and possible air-drying, they insist it's
not them.  We've checked pH on water, accurately calibrated the bluing
and acid/water washes (we stain regressively), and just about run out of
ideas.  I'm beginning to suspect the hematoxylin is leaving a
precipitate, or maybe the microtomy is inconsistent and ruining cell
integrity, or .... maybe it's terrorism!   Any ideas out there?

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