Re: [Histonet] Mouse Embryo Processing


It may be the type of paraffin you are using. Surgipath makes a great
infiltration medium for the processor. It is called infiltration medium.
The embedding medium would be EM 400. I would also add some time to the
paraffin - 45 min. x3.

Something else you may want to think about is using a xylene substitute
instead of xylene.

Hope this helps.


Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax

                      Stephanie Schmoll                                                                                                     
                      Sent by:                            cc:                                                                               
                      histonet-admin@lists.utsouth        Subject:  [Histonet] Mouse Embryo Processing                                      
                      10/14/2003 03:53 PM                                                                                                   

I am fairly new to tissue processing with paraffin, have always done
cryo-embedding in the past.  I am having trouble processing late stage
mouse embryos, day 17 and 18.  The paraffin doesn't seem to be
infiltrating.  Any suggestions would be appreciated.

My current protocol is as follows:  24hrs 10% NBF, 70% EtOH 30 min, 80%
30 min, 95% 30 min x2, 100% 30 min x2, Xylene 30 min x2, paraffin 30
min x3.

Stephanie Schmoll, Morphology Core Manager
Center for Organogenesis
University of Michigan Medical School
5795C  Medical Science Bldg.  II
Phone: (734) 647-9021
Fax: (734) 647-9559

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