I am fairly new to tissue processing with paraffin, have always done 
cryo-embedding in the past.  I am having trouble processing late stage 
mouse embryos, day 17 and 18.  The paraffin doesn't seem to be 
infiltrating.  Any suggestions would be appreciated.

My current protocol is as follows:  24hrs 10% NBF, 70% EtOH 30 min, 80% 
30 min, 95% 30 min x2, 100% 30 min x2, Xylene 30 min x2, paraffin 30 
min x3.


Stephanie Schmoll, Morphology Core Manager
Center for Organogenesis
University of Michigan Medical School
5795C  Medical Science Bldg.  II
Phone: (734) 647-9021
Fax: (734) 647-9559


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