Dear Bill

We have experienced uneven staining on our Ventana NexES stainer over the
past number of months. It is not confined to any particular antibody, and
does'nt happen all the time. We have been told by Ventana that it is a
worldwide problem with coated slides. Apparently a number of brands are
manufactured in the same factory. We use Starfrost slides from Knittel
Glaser in Germany.
As yet, working with the people from Ventana, and trying a variety of slide
brands, we have been unable to resolve the problem.
I would be interested in hearing from others that have encountered similar
problems, and what actions they have taken to resolve the issue.

Liam Brennan
Histopathology Dept
Belfast City Hospital
Northern Ireland

-----Original Message-----
From: Bill Sinai [mailto:bills@icpmr.wsahs.nsw.gov.au]
Sent: 01 October 2003 22:31
To: histonet (E-mail)
Subject: [Histonet] Superfrost Plus Slides




Dear All,

Has anyone had a problem with Superfrost Plus slides on automated
Immunohistochemistry Staining machines?  If so what have the problems been?
Also if the problem was resolved and how it was resolved.

We obtain our slides through a third party agency in Australia.  We are at
present using Menzel-Glaser (German) brand.  I am led to believe they are
the largest slide and coverglass manufacturer in the world.

Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774


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