[Histonet] DAKO ER and PR antibodies
Hi out there in Histonet land:
I am having a problem working up dilutions for DAKO's ER (M7047) and PR
I am using the Envision+ Detection system
Suggestions from DAKO were:
ER 1:100 to 1:150 with Envision+ and a 20 minute target retrieval
PR 1:200 to 1:400 with Envision+ and a 20 minute target retrieval.
I've gone less and more and still cannot get any where near the staining on
these cases that went out to a IHC Laboratory than I am using as a
Any suggestions would certainly be appreciated.
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