skull decalcification

From:Matt McElwee

Hi everyone.  Does anyone have a good way to decalcify mouse skulls that
doesn't shrink or otherwise alter the brain?  We want to section mouse
heads from neonates through 2 weeks of age.  I know that we'll have to
change the time that the brain is in the decalcifying solution,
depending on the age of the mouse, but if anyone can recommend a good
decalcifying solution or give any other advice, I'd really appreciate
Thank you.

Matt McElwee
Research Specialist
Department of Surgery
K4/617 Clinical Science Center
600 Highland Avenue
Madison, WI 53792
(608) 263-7648

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