Re: frozen sections revisited

From:Kathleen Spencer

For perfused brain, as soon as you pick up the section, place your 
finger under the section for just a second, and then air-dry the slides 
at room temp. We like charged slides. I store them in a slide box with 
some desicant in the -70 , if fresh frozen. Once they are fixed, or if 
they are perfused, I store them in the 4 degree fridge.

On Sunday, October 27, 2002, at 01:01 PM, wrote:

> Histonetters,
> I know this subject has been done on HISTONET before, but can we open 
> this
> up again.  how do you process frozen sections to get the best slide
> adhesion?
> do you fix immediately in cold fix in the cryostat or airdry and then 
> fix?
> what do you fix in, ie cold acetone,etc.?  do you always try to stain
> slides soon after preparing?  do you store FS for staining later?  how 
> are
> FS stored, ?wrapped individually in foil and plastic bags, etc.?  do you
> store them unfixed or fixed?  how are stored FS retrieved from the 
> freezer?
> Thanks for responding to this mini survey.
> Patsy Ruegg
> James,
> are you talking about cells in suspension or tissue sections lifting off
> silane coated slides?
> for keeping FS on plus slides, i pick them up and let them airdry well 
> for
> at least 10 min. at RT.  there are some different methods for what to do
> after drying to fix and stain right away or store frozen unfixed until
> ready to stain, but i think the initial drying at RT goes a long way to
> keep them on the slides.  some people have a jar of cold fixative in the
> cryostat and fix immediately after sectioning in the cryostat and then 
> air
> dry, but i have had trouble with preservation of morphology doing it 
> this
> way.  people have their favorite methods that work the best in their 
> hands.
> my WS partner said she had some antigenicity loss from storing dryed
> unfixed sections in the freezer, but i have always done it that way 
> with no
> apparent problems.
> Original Message:
> -----------------
> From:
> Date: Fri, 25 Oct 2002 11:52:45 -0400
> To:
> Subject:
> Has anyone had any problems with lifting off cells for LCM that have 
> been
> picked up on SuperFrost Plus Slides?  How
> did you resolve the problem?
> Also does anyone have any suggestions for keeping fresh frozen cyrostat
> sections on any kind of slide.
>                          Thanks,
>                              JPZ
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