Sandi,
There is a procedure for a Modified Brown and Brenn in the Theory and
Practice of Histotechnology book.  Do you have it?  The method uses
tartrazine.  Works great.  If you need it, let me know.

Rande Kline
EM Science


                                                                                                           
                      Snobird75@aol.com                                                                    
                                               To:       histonet@pathology.swmed.edu                      
                      10/24/2002 07:55         cc:                                                         
                      PM                       Subject:  Gram Stain                                        
                                                                                                           
                                                                                                           




Dear Friends:
Does someone have a procedure for doing a gram stain, where you don't use
the
50% solution of xylene and picric acid (Bouins).
I know there is a procedure out there. I just don't remember it.
Thanks for you help
SAndi Miller HT
USAMRICD Research
Maryland