RE: Re-embedding SPURR
Patsy is giving the best method for re-embedding. It may take a couple of
days and several changes of infiltration solution to reach a stage for
embedding again. Pam Marcum
> -----Original Message-----
> From: Patsy Ruegg [mailto:firstname.lastname@example.org]
> Sent: Tuesday, October 22, 2002 2:02 PM
> To: Fabien Fuente
> Cc: HistoNet Server
> Subject: Re: Re-enbedding SPURR
> what i do is cut away as much as the plastic as possible to try and expose
> the tissue and then start reinfiltrating as if it were fresh tissue and
> Fabien Fuente wrote:
> > Hello everyone,
> > We are using Spurr for a while on rather big samples (about 1cm3). After
> > several successful embedded samples, we had recently experienced some
> > troubles with our last samples.
> > The resin didnot polymerized well in the center of the sample. We end up
> > with gummy and soft resin in the center of the sample.
> > As these samples are valuable, did anyone know a technique to
> remove this
> > unpolymerized resin and re-embed the entire sample without losing the
> > samples.
> > Thank you.
> > Fabien Fuente
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