Gayle is correct!  JB-4 is GMA and is not recommended for IHC of any type.
Some success with very low molecular weight antibodies and systems have
shown results however they are rare and often not repeatable. Histochemistry
is possible and has worked with many enzymes.
Pamela A Marcum
Histology/Microscopy
Product Manager
Polysciences, Inc
400 Valley Road
Warrington, PA 18976
Telephone: 800-523-2575 Ext 167
           215-343-6484 Ext 167
Fax:       800-343-3291
           215-343-0214
E-mail: pmarcum@polysciences.com

> -----Original Message-----
> From: Gayle Callis [mailto:gcallis@montana.edu]
> Sent: Monday, October 14, 2002 6:11 PM
> To: histonet@pathology.swmed.edu
> Subject: TRAP,ect with JB4 or methylmethacrylate
>
>
> You wrote:
>
>
> Does anyone know if TRAP staining, Alk Phos staining, or LacZ staining
> (with Xgal) can be done on methylmethacrylate embedded (JB4 kit) calcified
> bone tissue?  If so, how did you fix and process the tissue?  Thanks
> again!
>
> Ryan
>
> The JB4 kit from Polysciences is not methylmethacrylate (MMA), but glycol
> methacrylate (GMA)  I know enzyme staining can be done with GMA but not
> sure about the ones you are asking for.
> I know that TRAP has been used by Sandy Marks Jr (this is in the
> literature) using GMA plastic.
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> 19th and Lincoln St
> Bozeman MT 59717-3610
>
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
>
> email: gcallis@montana.edu
>
>
>