Hi histocybernetters
I would like to get your opinions on procedures for cytology specimens
that are prepared for  immunocytochemistry. I am currently fixing direct
smears or cytospins immediately in 95% absolute alcohol for 10 mins .I
do this for any cytoplasmic or cell membrane antigens . I fix in
methanol:acetone 50:50 for 10 mins for any nuclear antigens such as Tdt
or Myo D1 (to increase permeabilisation)is this necessary?. I work in a
busy paediatric laboratory so we don't get a lot of cytology specimens
but on occasion we are requested to do them. My results to date have
been patchy especially following storage at -20C. I would like to be a
bit more confident about my procedures and would like  any comments from
anyone doing a lot of immunocytochemistry on cytologyy specimens . Also
how do you store your smears ? Do you spray fix or use PEG.?