Our engineering department had to inspect and test all equipment coming into
the lab.  It could be purchased outside as long as it met the electrical
specifications of the facility.

Jennifer MacDonald


----- Original Message -----
From: "Donna Carlton" 
To: "'HistoNet'" 
Sent: Wednesday, October 24, 2001 2:45 PM
Subject: RE: antigen retrieval


> Hello,
> I am sharing this information with all of you because the situation took
me
> by surprise.  I thought you might like to check with your engineering
> department, before purchasing a vegetable steamer.  Some places are more
> strict than others sooooo- take what you want and leave the rest:-)
>
> I bought a vegetable steamer for the Histology laboratory only to find out
> that I could not use it for medical purposes.  Our engineering department
> has very strict rules concerning safety, and fire issues.  I personally,
was
> a bit taken back that I could not use the steamer.  So, I purchased a
> "IsoTemp" water bath from Fisher Scientific.  It costs more but it meets
> safety recommendations for the laboratory that I work in.
>
> Hope that this is helpful.
> > -----Original Message-----
> > From: Morken, Tim [SMTP:tim9@cdc.gov]
> > Sent: Wednesday, October 24, 2001 4:45 AM
> > To: '=?UNKNOWN?Q?V=E9ronique?= Wunderle'; histonet@pathology.swmed.edu
> > Subject: RE: antigen retrieval
> >
> > Veronique,
> >
> > We use a steamer (20 minutes) to do our antigen retrieval, although we
use
> > the microwave to heat the AR solutions.
> >
> > Tim Morken
> > CDC, Atlanta
> >
> >
> > -----Original Message-----
> > From: =?UNKNOWN?Q?V=E9ronique?= Wunderle [mailto:vwunderle@yahoo.com]
> > Sent: Wednesday, October 24, 2001 3:37 AM
> > To: histonet@pathology.swmed.edu
> > Subject: antigen retrieval
> >
> >
> > hi everybody !
> > Being pretty new in the big world of
> > immunohistochemistry I am asking myself lots of
> > questions (some people tells me it's a good thing..).
> > I know that I should get a good book on the subject
> > (any advice ?) but I find that "real-life" experience
> > is often much more informative.
> > So the question of the day is about antigen retrieval.
> > I have been looking around for different protocols and
> > most of them use a microwave oven. I can't buy right
> > now a microwave especially for IHC and I am a bit
> > concerned about setting up parameters on an oven used
> > extensively for other purposes. I am a bit anxious
> > that the life span of this oven is going to be pretty
> > short...
> > So, I tried the heating plate protocol (10mn in
> > boiling citrate buffer) on brain sections. So far, it
> > seems to be working pretty well. But I may be lucky.
> > I am therefore wondering if people out there tried
> > this and what you think about it. What make you prefer
> > the microwave method and here is the ultimate question
> > : is a microwave oven a "must have" for doing
> > incredible IHC ?
> > all your advices are welcome...
> > veronique.
> >
> > __________________________________________________
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