-----Original Message-----
From: Kathy Paton 
Sent: Wednesday, October 25, 2000 9:26 AM
To: 'Cindy Burchell'
Subject: RE: chromotrope 2R for eosinophil granules


Dear Cindy
Carbol-chromotrope method for eosinphils (Lendrum 1944)
Reagent
Phenol crystals (melt by gentle heat).......1g
Chromotrope 2R     ........................0.5g
Mix
Distilled water    ........................100ml

This stain keeps for about three months

METHOD
Hx as desired
Place in staining solution for 30min
Rinse in water
DCM

In my experience this is very selctive.   Give it a go
Kathy Paton
Surgical Pathology Unit
North Shore Hospital
Auckland
New Zealand

-----Original Message-----
From: Cindy Burchell [mailto:burchell@facstaff.wisc.edu]
Sent: Wednesday, October 25, 2000 4:49 AM
To: histonet@pathology.swmed.edu
Subject: chromotrope 2R for eosinophil granules


	Hello Histonetters,
    It's been a while since I have had the chance to be in contact with 
you, and now I need a favor again.  My bosses insist that I use something 
called Chromotrope 2R to counterstain eosinophilic granules after IHC 
staining MBP of eos.  I've found references, but never any specific 
methods.  I would be in debt to anyone who could spell out just how to 
handle this counter stain.   Thanks so much in advance for your time if you 
will help me in this matter!!!   Cindy B.