Re: chromotrope 2R for eosinophil granules
<underline>The meyhod for Carbol chromotrope 2R stain is in "Manual of
Histiological Techniques and their Diagnostic Application by
Bancoft & Cook.
For those who can't get their hands on a copy of the book the
method is as follows.
Carbol Chromotrope 2R
</underline>Melt 1g of phenol in a flask under hot water, add 0.5g chromotrope
2R. Mix, dissolve resultant sludge in 100mL distilled water and
filter. The solution keeps for up to 3 months.
1.Bring sections to water
2.Stain nuclei with an alum haematoxylin, differentiate and blue
3.Stain in Carbol Chromotrope solution for 30mins ( or longer if
4.Wash, dehydrate, clear and mount
Eosinophol granules red
Red blood cells pale red
Technical Officer ( Scientific)
Institute of Forensic Medicine
Fax 61 2 95664573
<< Previous Message | Next Message >>