nerve teasing

From:Sue Danielson <sdaniels@post.its.mcw.edu>

Hi Jean,

I have always used a 50% aqueous solution of glycerin to tease the nerve in
and also to soak the nerve in before teasing (preferrably overnight).  I
find that this softens the nerve up, making it easier to tease after
osmication and rinsing.  We tease using the 3c fine point tweezers from EMS.
I have never used epon as a teasing media...cant comment on how it might
compare to the glycerin method.

Susan K. Danielson, MS
Neuromuscular Lab Coordinator
Dept. Neurology, Medical College of Wisconsin
ph: 414.259.3836
fax: 414.454.7905




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