At 08:00 AM 11/24/99 GMT0BST, RUSS ALLISON wrote:
>Both Barka & Anderson and Lillie give credit to Burstone.  I have  not seen
the paper which preceeded his book (1960), but judging  from the 1960
reference you gave John, I presume it was not in his  1959 publication in
the same Journal? (Burstone MS. New  histochemical techniques for the
demonstration of tissue oxidase  (Cytochrome oxidase). J Histo Cyto
1959;7:112-122)

  I've just dug out some old photocopies. The paper you quote doesn't seem
  to mention AEC, but the next one of the series does.  This is
  Burstone, M. S. 1960. Histochemical demonstration of cytochrome oxidase
  with new amine reagents. J. Histochem. Cytochem. 8: 63-70.
  He used AEC in conjunction with N-phenyl-p-phenylenediamine in a
  modified NADI reaction for cytochrome oxidase. A postulated formula
  for the product (a polymeric indamine dye) is given in the Discussion.
  This doesn't help with the peroxidase reaction though, because for
  that the chromogen is AEC alone.

  According to the Colour Index, 3-amino-9-ethylcarbazole is used in
  the synthesis of some oxazine dyes and pigments, but it is reacted with
  other organic compounds in circumstances very different from anything
  histochemical.