Elaine,
we use CD-41 from Dako (cat# M7057).  We use Dako's Proteinase K at room
temp for 10 minutes (cat# S3020).  We have  30-30-30 incubation times at
room temp.  Hope this helps.  Good luck.

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas


> -----Original Message-----
> From:	Elaine Dooley [SMTP:DOOLEEO@shands.ufl.edu]
> Sent:	Tuesday, November 23, 1999 2:27 PM
> To:	histonet@pathology.swmed.edu
> Subject:	cd41
>
> Histonetters,
>
> Does anyone out there have a good protocol for CD41 on formalin fixed
> paraffin embedded tissue that they would share?  In particular for bone
> marrow biopsies and clots.
>
> Elaine Dooley
> 352-395-0111 ext 5-3350
>
> Thanks in advance
>