FW: Insitu pcr

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From:Patsy.Ruegg@UCHSC.edu (by way of histonet)
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-----Original Message-----
From: Ruegg Patsy
Sent: Tuesday, November 23, 1999 1:57 PM
To: 'Deborah Moffett'
Subject: RE: Insitu pcr

You can try heating the slides overnight at 60 d C, but this is an common
problem with insitu pcr, the tissue just does not stand up to the  harsh pcr
cycles of heat and chemical enzymes.  It is a real obsticle to doing insitu
Patsy Ruegg

-----Original Message-----
From: Deborah Moffett [mailto:DebMoffett@moffett.swinternet.co.uk]
Sent: Tuesday, November 23, 1999 11:41 AM
To: histonet
Subject: Insitu pcr

We are attempting to do insitu PCR on gut and kidney paraffin sections for
bovine adenovirus. We are having some difficulty with disintegration of the
sections during the pcr cycles. Can anyone reccommend a solution to this
the sections are 4-5um thick , lifted on superfrost plus gold slides and
heated for one hour at 60C.

Deborah Moffett

Veterinary Sciences Division

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