Susan,
We don't do this procedure often, but when we have we have not seen a
background on the entire slide. I think you might if you use slides with
more coating. Again we use the 1/2x slides. Also the reference for the Sudan
IV procedure that I use is from the Medical College of Georgia Staining
Manual as I got it from a histotech I worked with at the University of
Georgia and she trained at MCG.
John

> ----------
> From: 	Susan Meloan[SMTP:smeloan@mail.mcg.edu]
> Sent: 	Friday, November 19, 1999 7:13 AM
> To: 	jtarpley@amgen.com; uvsgc@msu.oscs.montana.edu;
> histonet@pathology.swmed.edu
> Subject: 	Re: RE: crystalmount/oil red O/gentle jane slides
>
> John,
>
> I was also going to say that when used slides cut with the Instrumetics
> tape, we got a background staining on the whole slide.  We usually mount
> fat stains on plus slides also, and get a cleaner stain.
>
> We have been  using the Shandon Immuno Mount.  I have wondered about
> Crystal Mount, but have not tried it.
>
> Susan
>
> Susan Meloan
> Chief Histotechnologist
> Department of Pathology
> Medical College of Georgia
> Augusta, Ga 30912
> (706) 721-3630
> e-mail: smeloan@mail.mcg.edu
>
>
>
> >>> "Tarpley, John" <jtarpley@amgen.com> 11/18 2:42 PM >>>
>
>
> > Gayle Callis wrote:
> > Subject: 	crystalmount/oil red O/gentle jane slides
> >
> > I do not know the compatibility of Oil Red O and Crystalmount, I only
> use
> > the Gentle Jane slides for lung/undecalcified bone frozens for IHC,
> > or Beta Gal stained whole tissue mounts. All our frozens for Oil Red O,
> > and in general are just mounted on Plus charge slides, we reserve the
> > Gentle Jane for very special protocols, not a routine everytime usage.
> Recently we had an occasion to section some difficult tissues for fat
> staining and I tried the Instrumedics system. We use Sudan IV rather than
> Oil Red O and we found it worked well with the stain. We mounted the
> sections using Sandon's Immu-Mount water based mounting media. They didn't
> look as clean as normal slides and did show the "bubble look" appearance,
> but were still readable.
> 	-snip snip-
> > I think it is important to not use 4X coated slides, even 1 X coated
> > slides, we successfully use the 1/2X coated slides even for
> undecalcified
> > bone frozens, and this lessens the "bubble look" of the polymer after is
> > is UV  polymerized.  Tried the 4X again, and immediately went to the
> > 1/2X coated slides.
> I agree. We have the best results with 1/2x slides both for bone and soft
> tissue.
>
> 	-snip snip-
>
> > John Tarpley 15-2-B
> > Associate Scientist
> > Specialist Image Analysis & Immunohistochemistry
> > Amgen Inc
> > One Amgen Center Drive
> > Thousand Oaks, CA  91320
> >
> > Views expressed are mine alone and do not represent the views of my
> > employer
>
>