Re: Fluoro-Jade-staining
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of histonet) |
To: | histonet@histosearch.com |
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On Fri, 19 Nov 1999, Wilhelm Mosgoeller wrote:
> I am looking for a supplier of a fluorescent stain for degenerating nerve
> fibers which is called "fluoro-jade".
I've got a reprint of a paper by AJ Eish et al 1998
Synapse 30:329-333 with some photomicrographs. They
look much the same as sections stained with any anionic
dye (eosin, acid fuchsine etc), except that the fluorescence
shows rather longer dendrites than you see with
conventional microscopy of moribund neurons. This
paper does not identify a source of the "fluoro-jade"
they used, but it does refer to the paper by LC Schmued
et al (1997) Brain Res 751: 37-46 in which this
fluorochrome was introduced. I haven't seen that one,
but surely it must say where the stuff was bought or
how it was made. If it's a mysterious secret, don't
use it! (Eosin and acid fuchsine are both fluorescent.)
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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