Re: Fluoro-Jade-staining

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From:"J. A. Kiernan" <> (by way of histonet)
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On Fri, 19 Nov 1999, Wilhelm Mosgoeller wrote:

> I am looking for a supplier of a fluorescent stain for degenerating nerve
> fibers which is called "fluoro-jade".

   I've got a reprint of a paper by AJ Eish et al 1998
   Synapse 30:329-333 with some photomicrographs. They
   look much the same as sections stained with any anionic
   dye (eosin, acid fuchsine etc), except that the fluorescence
   shows rather longer dendrites than you see with
   conventional microscopy of moribund neurons. This
   paper does not identify a source of the "fluoro-jade"
   they used, but it does refer to the paper by LC Schmued
   et al (1997) Brain Res 751: 37-46 in which this
   fluorochrome was introduced. I haven't seen that one,
   but surely it must say where the stuff was bought or
   how it was made. If it's a mysterious secret, don't
   use it! (Eosin and acid fuchsine are both fluorescent.)

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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