RE: Color My World
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From: | Michael Archambault <marchambault@osiristx.com> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Reviewing an older text regarding museum preparation of specimens, I ran
across the following procedural reference:
Wash the specimens in running water to remove any residual fixative
Transfer to 95% alcohol, anywhere from 1/2 to 12 hours- watch the
specimen carefully;
the alcohol will bring back the color, but if left too long
it will fade away (irreversible)
Remove from alcohol, blot dry and photograph
Transfer to a storage medium (1416g sodium acetate, 4L glycerine,
bring to 10L final volume)
for long term display- color won't last forever, and sample
will float until it is fully permeated
with the glycerine solution.
Otherwise, I would think you could process the specimen after photography
(keeping in mind that it may not be fully alcohol dehydrated during the
color development- it may need more time after photographing).
Let me know if it works
Mike
Michael P Archambault
Research Scientist
Bone and Soft Tissue Program
Osiris Therapeutics, Inc
2001 Aliceanna St
Baltimore, MD 21231
> ----------
> From: greg tesdall[SMTP:gtesdall@yahoo.com]
> Sent: Friday, November 19, 1999 10:02 AM
> To: HistoNet Server
> Subject: Color My World
>
> I have some 10% formalin well fixed tissue that now
> has no color. A Pathologist wants to photograph the
> tissue and remembers hearing of soaking it in
> something that will bring some color back. Any help?
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