FW: Color My World
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From: | Michael Archambault <marchambault@osiristx.com> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
> Reviewing an older text regarding museum preparation of specimens, I ran
> across the following procedural reference:
> Wash the specimens in running water to remove any residual fixative
> Transfer to 95% alcohol, anywhere from 1/2 to 12 hours- watch the
> specimen carefully;
> the alcohol will bring back the color, but if left too long
> it will fade away (irreversible)
> Remove from alcohol, blot dry and photograph
> Transfer to a storage medium (1416g sodium acetate, 4L glycerine,
> bring to 10L final volume)
> for long term display- color won't last forever, and sample
> will float until it is fully permeated
> with the glycerine solution.
>
> Otherwise, I would think you could process the specimen after photography
> (keeping in mind that it may not be fully alcohol dehydrated during the
> color development- it may need more time after photographing).
>
> Let me know if it works
>
> Mike
>
> Michael P Archambault
> Research Scientist
> Bone and Soft Tissue Program
> Osiris Therapeutics, Inc
> 2001 Aliceanna St
> Baltimore, MD 21231
>
>
>
> ----------
> From: greg tesdall[SMTP:gtesdall@yahoo.com]
> Sent: Friday, November 19, 1999 10:02 AM
> To: HistoNet Server
> Subject: Color My World
>
> I have some 10% formalin well fixed tissue that now
> has no color. A Pathologist wants to photograph the
> tissue and remembers hearing of soaking it in
> something that will bring some color back. Any help?
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