embryo/age/calcification

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From:Gayle Callis <uvsgc@msu.oscs.montana.edu> (by way of histonet)
To:histonet@histosearch.com
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A whole new era in my lab.  How much calcification is  present
in a 3 day old mouse?  also a newborn mouse?  Our transgenic mouse
expert has specimens sitting in formalin, collected, frozen, allowed to
fix in formalin while thawing.  We would like to process the
whole mouse in all instances, and wondered how much decalcification
would be necessary, if needed at all, preferrably with 10% formic
acid.

Gayle Callis




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