Permeabilization with ethanol (Whew! What a mouthful)

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I am looking for a reference for using buffered, graded ethanols for membrane
permeabilization that is a little less destructive than saponin or other
methods. A detailed method would suffice or the exact reference you use.  Any
and all replies will be appreciated.
Thanks in advance,
Nancy Lemke
Henry Ford Hospital
Dept. of Neurosurgery
Detroit, MI

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