If it is any benefit I agree with this message.... my tissue is rat brain
I have used glycerin in the past but found no benefit...Mary
On Fri, 29 Oct 1999, Geoff McAuliffe wrote:

> denise M m Long-Woodward wrote:
>
> > Hi! I'm looking for instructions on how to "cryopreserve" mouse tissue
> > using sucrose and OCT. Details will be appreciated.  Many thanks for your
> > insight.
>
> Denise:
>
>     After fixation and several buffer washes, I soak the tissue in cold 10%
> sucrose in buffer until it sinks,usually 1-3 hours depending on size. Then
> cold 20% sucrose in buffer until it sinks which is usually overnight. I have
> used concentrations of sucrose as high as 30% but see no benefit. I only  use
> OCT to "glue" the tissue to the microtome stage.
>     Rapid freezing of the tissue is very important to prevent ice crystal
> artifacts!
>
> Good luck,
> Geoff
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> mcauliff@umdnj.edu
> **********************************************
>
>
>
>