charged slides

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From:Ms Louise Taylor <179LOU@chiron.wits.ac.za> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Hi histonetters,

We are experiencing a problem with charged slides and "Declere"
solution from Cellmarque. We find that a large proportion of tissue
sections (formalin fixed, paraffin embedded) exposed to microwave
pretreatment with this solution are washing off.
("Declere" is purported to be a combination dewaxing, rehydrating and
antigen retrieval solution)
Has anyone else had this problem and is there a way to circumvent it?

All comments will be welcome
many thanks
Louise Taylor
SAIMR
Johannesburg
South Africa




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