HistoNet@Pathology.swmed.edu wrote:
Hello Histonetters,
I would like to do fluorescence in situ hybridization on paraffin embedded
sections using RNA probes.  I would appreciate it very much if anybody who
has worked on this could share the protocol with me.  Thanks in advance.

Agripina


Agripina C. Suarez
Cardiovascular Research Laboratory
McDonald Research Wing, St. Paul's Hospital
1081 Burrard St., Vancouver
B.C., Canada V6Z 1Y6
Phone: (604) 631-5659
Fax:   (604) 631-5351

Hi Agripina,

I would suggest the Hyb-Probe system from Shandon Lipshaw
Cat.#502100 this system utilizesFluorescein-labeled nucleic acid probes.
This system allow identification of specific nucleic acids DNA and RNA.
This system is very time efficient and sensitive. Good luck and I Hope this
info. helps.

Sincerely,
Carol Tucker-Burden
Emory University
Atlanta, GA.
Dept. Of Surgery
Transplant Immunology Research Ctr.

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