Patterson, Noelle wrote:

> I am hoping you can help.  There have been several discussions on image
> analysis on the Histonet before.  I realize this question may be a little
> different, but we are about to embark on a new protocol.  See our
> technicians question below.  Dr. Ring is not on Histonet, but I will happily
> pass on any correspondence.
>
> For those of you who like more background:
>
>  Traditionally, we have gone through a long days procedure to procure fresh
> mouse islets from several murine pancreata, as a source to do islet
> transplants.  We are looking for better ways to assess the islet size,
> condition, and speed up islet isolation while improving islet recovery (this
> is an ongoing goal for years now, really).  Because islets can be easily
> picked (in media placed in a 60mm-100mm tissue culture/petri dish) using
> particular dyes, we would like to utilize those dyes, and light microscopy
> to determine islet size and viability.  Some of these islets are further
> digested for FACS Analysis.
>
> Any ideas, comments, and further questions are welcomed.
>
> Sincere Thanks,
> Noelle
>
> PS If anyone has the archives of previous discussions, or the dates so I can
> request the archives from Herb, please let me know.  Of course, I recently
> deleted these thinking our department would never spring for new equipment.
> Little did I know!
> > ----------
> > From:         Ring, Michael
> > Sent:         Tuesday, November 17, 1998 9:03 PM
> > To:   Patterson, Noelle
> > Subject:      histo list question
> >
> > Here is the question. Please feel free to edit as you see fit. I do not
> > know the proper protocol for the signature, so below is my best guess.
> > Thanks!
> >
> > --------------------------------------
> >
> > Our group is considering setting up a digital microscopy station. The
> > purpose is to automatically analyze images of pancreatic islet cell
> > preparations. After cell preparation, the computer needs to:
> > 1. acquire the image (light only, no fluorescence)
> > 2. clean up the image
> > 3. isolate the relevant cell clusters using brightness or color
> > thresholding
> > 4. count and measure the resulting binary objects
> > 5. maintain a database of images and data
> >
> > Does anybody have any recommendations on cameras and software?
> >
> > I have been considering the Pixera camera as a reasonable low cost
> > digitial camera.
> >
> > Software possibilities include Metamorph (Universal Imaging), ImagePro
> > (Media Cybernetics), NIH Image (NIH), Visilog (Noesis), and Visual Basic
> > (rolling our own).
> >
> > If anybody has had good or bad experiences in this area I would be very
> > interested.
> >
> > Thanks.
> >
> > Michael Ring
> > c/o Noelle Patterson
> > Immune Cell Biology Program
> > Naval Medical Research Center
> >
> >
> >
> > Dr. Michael Ring
> > Computer Coordinator for the
> > Immune Cell Biology Program
> > Naval Medical Research Institute
> > Bethesda, MD
> > ringm@nmripo.nmri.nnmc.navy.mil
> >

  Hi ,

coincidently I was in a workshop about a new digital imaging software of SIS (
Soft Imaging System GmgH) .
Their new software  called "analySIS" looks very  good.
It's  MSWindows conform ... means all output is compatible to Word, Exel, etc.
.
They said that a normal workstation with a framegrabber and a 3chip CCD camera
is enough on the hardware-side.
The software includes a Access compatble database and some really interestin
gimmicks .
The software and ...if you want... the hardware are distributed by Olympus
Optical .

Hope, you'll find the right stuff

Joachim Siegmund


BTA
Hamburg,Germany