Beth,
	Kirkegard and Perry make a product called contrast red. We tried it
here and it was considered suboptimal for nuclear detail. Also tried methyl
green.
Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov

> ----------
> From: 	broche@wlgore.com[SMTP:broche@wlgore.com]
> Sent: 	Tuesday, November 24, 1998 3:09 PM
> To: 	Histonet@pathology.swmed.edu
> Subject: 	Counterstain
>
> Histonetters:
>
> We are "staining" frozen sections of B-gal. cells to end up with a
> turquoise-blue reaction product.  The color is soluble in alcohols and
> clearants.  We use crystal mount to permanently mount.  We are looking for
> a counterstain that will not obscure the turquoise-blue color and will not
> leach out into the aqueous mounting media.  So far our hematoxylin
> (Mayers)
> is too dark, and neutral and nuclear fast red bleed out into the mountant.
> Anyone have any suggestions?  I sort of remember discussions of this
> nature
> in the past but , of course, didn't archive them at the time.
>
> Thanks for the help,
>
> Beth Roche
> Gore Hybrid Technologies
> Flagstaff, AZ
> broche@wlgore.com
>
>
>