Pigmented retinal epithelium

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From:Joyce Bousquet <jjbous@diacrin.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
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I am currently working with 5 microns paraffin sections of  "pig" pigmented
retinal epithelium.  I have bleached the sections with 10% Hydrogen Peroxide
for 2 days and performed ISH on sections with a pig specific DNA probe to
test conditions and have had good results with ISH.  However, I am really
interested in using a Pig Y DNA  Probe on these sections and I am not having
any success.  Does anyone have any thoughts on why the Pig Y probe is not
working in this tissue?  (I have used this probe in other pig male tissue
and have had good results).

Joyce Bousquet
Diacrin, Inc.
Boston, MA

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