ER/PR- Cytoplasmic Staining Artifact

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From:Daniel & Linda Botsford <> (by way of histonet)
To:histonet <>
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What are the possible causes of cytoplasmic staining when doing
immunoperoxidase Estrogen Receptor staining? The controls show nice
crisp nuclear staining and no background staining. The specimen was a
needle biopsy sent from the X-ray Department. Is it possible to get
antigen diffusion from the nucleus to the cytoplasm due to a delay in
fixation or high antigen level?

Windsor Regional Hospital,
Windsor, Ontario, Canada.

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