RE: bone marrow trephines

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From:Alex Brown <> (by way of histonet)
To:histonet <>
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Hi Louise,
	We used to use RDC for about an hour or two for our trephines,
but it seemed to have an adverse affect on any  immunocytochemistry we
had to carry out on them. Now we use formal citrate, normally overnight
at room temperature.. If we receive the biopsy in the morning, it's
'demineralised' ( I still prefer the term decal'd, but I don't want to
re-open that issue ) overnight, processed the following night and
reported the next day. So that's about 3 days for turnaround time. We
could probably process it during the 2nd day and cut it in the evening,
but there wouldn't be much point, as there wouldn't be a pathologist
available to report it.
		Hope this helps,
			Alex Brown
			Crosshouse Hospital
			Kilmarnock, Scotland.
From: Ms Louise Taylor
Subject: bone marrow trephines
Date: 12 November 1998 17:00

Hi everybody,

A colleague who is being harrassed by clinicians and pathologists
alike has asked me to post this.
What are the preferences for demineralising bone marrow trephines?
How long are samples demineralised and at what temperature?
What is the embedding medium used - wax or GMA/PMA?
and what is the acceptable turn around time for such samples?
I would appreciate your resonse on this.

Many thanks
Louise Taylor
South Africa

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