Histonetters: I do manual H&E staining on human and animal tissue.
Lately some sections or parts of sections are not taking up the Eosin.
This problem is very intermittent, but very noticeable on slide review.
Sometimes the first level on a slide will stain great and on the third
level the whole right half of the section will not stain.  I've tried
drying the slides longer, alternating the side holder from side to side,
to make sure the paraffin melts correctly.  Any ideas?  Thanks in
advance, pplumlee@thermolase.com