RE: Superfrost slides

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From:Lab account <noreen@ADMCORP.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Kathy, try Plus CHARGED slides. I think the ones you have are coated with
poly-l-lysine.
Noreen

-----Original Message-----
From: KathY Liucci [mailto:KLIUCCI@lhsnet.com]
Sent: Friday, November 06, 1998 1:04 PM
To: Histonet@Pathology.swmed.edu
Subject: Superfrost slides


Hello:
I was wondering if anyone has had this problem.  We have superfrost plus
slides, with the coating being pink.  We use these for special stains.  The
slides are purchased from Allegiance but come with no label, so we do not
know who manufactures these slides.
The problem is this:  our control slides are not staining well with these
pink slides.  We especially noticed this with our Masson trichrome and our
retic stains.  When we take the slides out of water, there is a sheeting off
of the water, like what happens when you go from alcohol that has some water
in it, into xylene.  So we did an experiment with putting the control on our
regular white, superfrost slides and did the same special stains.  We
noticed a big improvement.  They stained very well.  So after all this, my
question is, could it be possible that these slides are coated or charged
too much and are actually diminishing the staining?  Any information will be
appreciated.
Thank you
Kathy Liucci




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