Pam,
	I am unclear as to what you mean by levels. Same section on
different place of the slide or different level from block? Also is it
only the Eosin or is the hematoxylin the same? If it is the same section
on a different position of the slide maybe the level of one of your
reagents is low. If it is from a different level in the block maybe it
is inadequate processing. Good Luck!!
Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov

> ----------
> From: 	Pam Plumlee[SMTP:PPlumlee@thermolase.com]
> Sent: 	Thursday, November 12, 1998 10:50 AM
> To: 	'histonet@pathology.swmed.edu'
> Subject: 	Manual H&E staining problem
>
> Histonetters: I do manual H&E staining on human and animal tissue.
> Lately some sections or parts of sections are not taking up the Eosin.
> This problem is very intermittent, but very noticeable on slide
> review.
> Sometimes the first level on a slide will stain great and on the third
> level the whole right half of the section will not stain.  I've tried
> drying the slides longer, alternating the side holder from side to
> side,
> to make sure the paraffin melts correctly.  Any ideas?  Thanks in
> advance, pplumlee@thermolase.com
>